Method for the isolation of highly purified Salmonella flagellins
نویسندگان
چکیده
منابع مشابه
Method for the isolation of highly purified Salmonella flagellins.
Ten different Salmonella serotypes were grown in a chemically defined medium supplemented with 0.01% yeast extract. After sedimentation of the cells by centrifugation, flagella were detached by exposure to pH 2 for 30 min at room temperature. The flagellaless cells were removed by centrifugation, and the flagellin in the supernatant was further purified by high-speed centrifugation, ammonium su...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1985
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.22.6.1040-1044.1985